Add to ORKGPremise of the study: Here we present a series of protocols for RNA extraction across a diverse array of plants; we focus on woody, aromatic, aquatic, and other chemically complex taxa. Methods and Results: Ninety-one taxa were subjected to RNA extraction with three methods presented here: (1) TRIzol/TURBO DNA-free kits using the manufacturer\u27s protocol with the addition of sarkosyl; (2) a combination method using cetyltrimethylammonium bromide (CTAB) and TRIzol/sarkosyl/TURBO DNA-free; and (3) a combination of CTAB and QIAGEN RNeasy Plant Mini Kit. Bench-ready protocols are given. Conclusions: After an iterative process of working with chemically complex taxa, we conclude that the use of TRIzol supplemented with sarkosyl and the TURBO D...
Plant DNA and RNA extraction methods are well established, with a wide range of protocols, depending...
Background: RNA extraction from tree species like Eucalyptus is very tedious and difficult task. In ...
Modern genotyping techniques, such as SNP analysis and genotyping by sequencing (GBS), are hampered ...
Resurrection plant Ramonda serbica is a suitable model to investigate mechanisms of desiccation tole...
ABSTRACT Functional genomic analyses require intact RNA; however, Passiflora edulis leaves are rich...
Many experiments in modern plant molecular biology require the processing of large numbers of sample...
Abstract View references (4)Background: Standard molecular biological methods involve the analysis o...
The RNAzol RT reagent was used to provide pure RNA from human cells. We develop a protocol using RNA...
Background: Many experiments in modern plant molecular biology require the processing of large numbe...
Abstract Background High-purity RNA serves as the basic requirement for downstream molecular analysi...
Background: There is a growing interest in transcriptomics studies parallel to the advancement of tr...
The isolation of high quality RNA is a crucial technique in plant molecular biology. The quality of ...
Isolation of high-quality and nondegraded RNA is a prerequisite for many modern molecular biology ap...
There are many protocols used for RNA extraction from plants, which vary according to the compositio...
Modern genotyping techniques, such as SNP analysis and genotyping by sequencing (GBS), are hampered ...
Plant DNA and RNA extraction methods are well established, with a wide range of protocols, depending...
Background: RNA extraction from tree species like Eucalyptus is very tedious and difficult task. In ...
Modern genotyping techniques, such as SNP analysis and genotyping by sequencing (GBS), are hampered ...
Resurrection plant Ramonda serbica is a suitable model to investigate mechanisms of desiccation tole...
ABSTRACT Functional genomic analyses require intact RNA; however, Passiflora edulis leaves are rich...
Many experiments in modern plant molecular biology require the processing of large numbers of sample...
Abstract View references (4)Background: Standard molecular biological methods involve the analysis o...
The RNAzol RT reagent was used to provide pure RNA from human cells. We develop a protocol using RNA...
Background: Many experiments in modern plant molecular biology require the processing of large numbe...
Abstract Background High-purity RNA serves as the basic requirement for downstream molecular analysi...
Background: There is a growing interest in transcriptomics studies parallel to the advancement of tr...
The isolation of high quality RNA is a crucial technique in plant molecular biology. The quality of ...
Isolation of high-quality and nondegraded RNA is a prerequisite for many modern molecular biology ap...
There are many protocols used for RNA extraction from plants, which vary according to the compositio...
Modern genotyping techniques, such as SNP analysis and genotyping by sequencing (GBS), are hampered ...
Plant DNA and RNA extraction methods are well established, with a wide range of protocols, depending...
Background: RNA extraction from tree species like Eucalyptus is very tedious and difficult task. In ...
Modern genotyping techniques, such as SNP analysis and genotyping by sequencing (GBS), are hampered ...
