Intracellular Nitric Oxide Measurement in Human Sperm Using 4, 5-DiaminoFluorescein-2-Diacetate and Flow Cytometry

Sharma, R.
Hani, S. Bani
Bayachou, Mekki
Sabanegh, E.
Agarwal, A.

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Publication date

September 2011

DOI

10.1016/j.fertnstert.2011.07.892

Publisher

Elsevier BV

ISSN

0015-0282

Abstract

Objective: Nitric oxide (NO) has a major contribution in male reproduction, especially in sperm motility. Methods for direct measurement of NO in single cell such as spermatozoa are complex, lack sensitivity, and are non-specific. The objective of this study was to standardize the measurement of intracellular nitric oxide in human spermatozoa using NO specific probe 4,5-diaminofluorescein-2-diacetate (DAF-2DA) and flow cytometry

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Intracellular Nitric Oxide Measurement in Human Sperm Using 4, 5-DiaminoFluorescein-2-Diacetate and Flow Cytometry

Abstract

Extracted data

Intracellular Nitric Oxide Measurement in Human Sperm Using 4, 5-DiaminoFluorescein-2-Diacetate and Flow Cytometry

Abstract

Extracted data

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