This report examines the molecular mechanism by which high-fidelity DNA polymerases select nucleotides during the replication of an abasic site, a non-instructional DNA lesion. This was accomplished by synthesizing several unique 5-substituted indolyl 2′-deoxyribose triphosphates and defining their kinetic parameters for incorporation opposite an abasic site to interrogate the contributions of π-electron density and solvation energies. In general, the Kd, app values for hydrophobic non-natural nucleotides are ∼10-fold lower than those measured for isosteric hydrophilic analogs. In addition, kpol values for nucleotides that contain less π-electron densities are slower than isosteric analogs possessing higher degrees of π-electron density. Th...
Translesion DNA synthesis, the ability of a DNA polymerase to misinsert a nucleotide opposite a dama...
High fidelity DNA polymerases maintain genomic fidelity through a series of kinetic steps that inclu...
The vast majority of DNA polymerases use the complementary templating strand of DNA to guide each nu...
This report describes the use of several isosteric non-natural nucleotides as probes to evaluate the...
The promutagenic process known as translesion DNA synthesis reflects the ability of a DNA polymerase...
DNA is a remarkable macromolecule that functions primarily as the carrier of the genetic information...
Translesion DNA synthesis (TLS) allows DNA polymerases to incorporate nucleotides opposite and beyon...
Translesion DNA synthesis (TLS) is the ability of DNA polymerases to incorporate nucleotides opposit...
Translesion DNA synthesis (TLS) allows DNA polymerases to incorporate nucleotides opposite and beyon...
O6-methylguanine is a miscoding DNA lesion arising from the alkylation of guanine. This report uses ...
DNA polymerases catalyze the insertion of a nucleoside triphosphate into the growing polymer chain u...
This report describes the use of several isosteric non-natural nucleotides as probes to evaluate the...
Translesion DNA synthesis, the ability of a DNA polymerase to misinsert a nucleotide opposite a dama...
Most models accounting for the efficiency and fidelity of DNA polymerization invoke the use of eithe...
Most models accounting for the efficiency and fidelity of DNA polymerization invoke the use of eithe...
Translesion DNA synthesis, the ability of a DNA polymerase to misinsert a nucleotide opposite a dama...
High fidelity DNA polymerases maintain genomic fidelity through a series of kinetic steps that inclu...
The vast majority of DNA polymerases use the complementary templating strand of DNA to guide each nu...
This report describes the use of several isosteric non-natural nucleotides as probes to evaluate the...
The promutagenic process known as translesion DNA synthesis reflects the ability of a DNA polymerase...
DNA is a remarkable macromolecule that functions primarily as the carrier of the genetic information...
Translesion DNA synthesis (TLS) allows DNA polymerases to incorporate nucleotides opposite and beyon...
Translesion DNA synthesis (TLS) is the ability of DNA polymerases to incorporate nucleotides opposit...
Translesion DNA synthesis (TLS) allows DNA polymerases to incorporate nucleotides opposite and beyon...
O6-methylguanine is a miscoding DNA lesion arising from the alkylation of guanine. This report uses ...
DNA polymerases catalyze the insertion of a nucleoside triphosphate into the growing polymer chain u...
This report describes the use of several isosteric non-natural nucleotides as probes to evaluate the...
Translesion DNA synthesis, the ability of a DNA polymerase to misinsert a nucleotide opposite a dama...
Most models accounting for the efficiency and fidelity of DNA polymerization invoke the use of eithe...
Most models accounting for the efficiency and fidelity of DNA polymerization invoke the use of eithe...
Translesion DNA synthesis, the ability of a DNA polymerase to misinsert a nucleotide opposite a dama...
High fidelity DNA polymerases maintain genomic fidelity through a series of kinetic steps that inclu...
The vast majority of DNA polymerases use the complementary templating strand of DNA to guide each nu...