The Reaction of Simple Antigens with Purified Antibody

In the present paper we report the results of experiments on the interaction of simple precipitating antigens with purified antibody solutions. It has been found that the behavior of the simple antigens with purified antibody solutions is somewhat different from that with antiserum, the difference apparently being due to a rather strong non-specific combination of the dye molecules with the constituents of serum not present in purified antibody, presumably mainly albumin. The effect of this combination seems to be to reduce the concentration of the free dye molecules to such an extent that in serum solution they react mainly in the monomeric form