We describe the construction and use of two classes of cDNA cloning vectors. The first class comprises the ΛEXLX(+) and λEXLX(−) vectors that can be used for the expression in Escherichia coli of protein encoded by cDNA inserts is achived by the fusion of cDNA open reading frames to the T7 gene 10 promoter and protein-coding sequences. The second class, the λSHLX vectors, allows the generation of large amounts of single-stranded DNA or synthetic cRNA that can be used in subtractive hybridization procedures. Both classes of vectors are designed to allow directional cDNA cloning with non-enzymatic protection of internal restriction sites. In addition, they are designed to facilitate conversion from phage λ to plasmid clones using a genetic me...
The ability to efficiently and reliably transfer genetic circuits between the key synthetic biology ...
Gene targeting refers to the precise modification of a genetic locus using homologous recombination....
For promoter studies the cloning, subcloning and transfer to different plasmid vectors usually requi...
We describe the construction and use of two classes of cDNA cloning vectors. The first class compris...
This paper describes the construction and characterization of a family of λ phage cDNA cloning vecto...
A multipurpose vector vas constructed which can be used for cloning DNA fragments of about 20 kb gen...
We have developed a fast and efficient procedure for generating cDNA libraries in plasmid or phage l...
I describe the construction of a variety of Escherichia coli recA deletion strains designed to facil...
The aim of this work was to develop a more efficient and usable basic form of cloning vehicle for ge...
Use of Escherichia coli bacteria as a host for high-level expression of cloned genes has become comm...
The versatility of insertional inactivation of beta-galactosidase activity for subcloning and sequen...
Phage-based Escherichia coli homologous recombination systems have recently been developed that now ...
The method uses a novel plasmid vector, p9lox5, containing a site-specific recombination sequence lo...
Plasmid DNA (pDNA) vectors are the current conventional technology driving therapeutic gene transfer...
3 p.-1 fig.We have constructed two plasmid vectors for cloning and expression of DNA fragments contr...
The ability to efficiently and reliably transfer genetic circuits between the key synthetic biology ...
Gene targeting refers to the precise modification of a genetic locus using homologous recombination....
For promoter studies the cloning, subcloning and transfer to different plasmid vectors usually requi...
We describe the construction and use of two classes of cDNA cloning vectors. The first class compris...
This paper describes the construction and characterization of a family of λ phage cDNA cloning vecto...
A multipurpose vector vas constructed which can be used for cloning DNA fragments of about 20 kb gen...
We have developed a fast and efficient procedure for generating cDNA libraries in plasmid or phage l...
I describe the construction of a variety of Escherichia coli recA deletion strains designed to facil...
The aim of this work was to develop a more efficient and usable basic form of cloning vehicle for ge...
Use of Escherichia coli bacteria as a host for high-level expression of cloned genes has become comm...
The versatility of insertional inactivation of beta-galactosidase activity for subcloning and sequen...
Phage-based Escherichia coli homologous recombination systems have recently been developed that now ...
The method uses a novel plasmid vector, p9lox5, containing a site-specific recombination sequence lo...
Plasmid DNA (pDNA) vectors are the current conventional technology driving therapeutic gene transfer...
3 p.-1 fig.We have constructed two plasmid vectors for cloning and expression of DNA fragments contr...
The ability to efficiently and reliably transfer genetic circuits between the key synthetic biology ...
Gene targeting refers to the precise modification of a genetic locus using homologous recombination....
For promoter studies the cloning, subcloning and transfer to different plasmid vectors usually requi...