Add to ORKGThe development of microfluidic tools for high-throughput nucleic acid analysis has become a burgeoning area of research in the post-genome era. Here, we have developed a microfluidic chip to perform 72 parallel 450-pL RT-PCRs. We took advantage of Taqman hydrolysis probe chemistry to detect RNA templates as low as 34 copies. The device and method presented here may enable highly parallel single cell gene expression analysis
Understanding biological complexity arising from patterns of gene expression requires accurate and p...
<div><p>We present a microfluidic device for rapid gene expression profiling in single cells using m...
Absolute, precise quantification methods expand the scope of nucleic acids research and have many pr...
The development of microfluidic tools for high-throughput nucleic acid analysis has become a burgeon...
Transcription measurements with single cell resolution are critical to understanding variable respon...
The first lab-on-chip system for picoliter droplet generation and PCR amplification with real-time f...
The Polymerase Chain Reaction (PCR) is an enzyme-based technique to amplify a single or few copies o...
The first lab-on-chip system for picoliter droplet generation and RNA isolation, followed by reverse...
Methods for single-cell analysis are critical to revealing cell-to-cell variability in biological sy...
The work presented here describes the development of a single integrated microfluidic device for RNA...
The behaviors of complex biological systems are often dictated by the properties of their heterogene...
Droplet microfluidics can identify and sort cells using digital reverse transcription polymerase cha...
Thesis (Ph.D.)--University of Washington, 2017-03In the last four decades, advancements in technolog...
Understanding biological complexity arising from patterns of gene expression requires accurate and p...
A microfluidic device for performing single copy, emulsion Reverse Transcription Polymerase Chain Re...
Understanding biological complexity arising from patterns of gene expression requires accurate and p...
<div><p>We present a microfluidic device for rapid gene expression profiling in single cells using m...
Absolute, precise quantification methods expand the scope of nucleic acids research and have many pr...
The development of microfluidic tools for high-throughput nucleic acid analysis has become a burgeon...
Transcription measurements with single cell resolution are critical to understanding variable respon...
The first lab-on-chip system for picoliter droplet generation and PCR amplification with real-time f...
The Polymerase Chain Reaction (PCR) is an enzyme-based technique to amplify a single or few copies o...
The first lab-on-chip system for picoliter droplet generation and RNA isolation, followed by reverse...
Methods for single-cell analysis are critical to revealing cell-to-cell variability in biological sy...
The work presented here describes the development of a single integrated microfluidic device for RNA...
The behaviors of complex biological systems are often dictated by the properties of their heterogene...
Droplet microfluidics can identify and sort cells using digital reverse transcription polymerase cha...
Thesis (Ph.D.)--University of Washington, 2017-03In the last four decades, advancements in technolog...
Understanding biological complexity arising from patterns of gene expression requires accurate and p...
A microfluidic device for performing single copy, emulsion Reverse Transcription Polymerase Chain Re...
Understanding biological complexity arising from patterns of gene expression requires accurate and p...
<div><p>We present a microfluidic device for rapid gene expression profiling in single cells using m...
Absolute, precise quantification methods expand the scope of nucleic acids research and have many pr...