The Saccharomyces cerevisiae amber suppressor tRNA gene SUP53 (a tRNALeu3 allele) was used to investigate the role of intron structure and sequence on precursor tRNA splicing in vivo and in vitro. This gene encodes a pre-tRNA which contains a 32-base intervening sequence. Two types of SUP53 intron mutants were constructed: ones with an internal deletion of the natural SUP53 intron and ones with a novel intron. These mutant genes were transcribed in vitro, and the end-processed transcripts were analyzed for their ability to serve as substrates for the partially purified S. cerevisiae tRNA endonuclease and ligase. The in vitro phenotype of these mutant RNAs was correlated with the in vivo suppressor tRNA function of these SUP53 alleles after ...
Aminoacyl tRNA synthetases (aaRS) are enzymes that add particular amino acids to cognate tRNAs. Thes...
The process of gene splicing, which involves the excision of introns from a pre-mRNA and joining of ...
AbstractStrains of Saccharomyces cerevisiae have been constructed that possess temperature-sensitive...
The Saccharomyces cerevisiae amber suppressor tRNA gene SUP53 (a tRNALeu3 allele) was used to invest...
The Saccharomyces cerevisiae leucine-inserting amber suppressor tRNA gene SUP53 (a tRNALeu3 allele) ...
In vitro mutagenesis was used to create putative opal suppressor alleles of a tRNATrp gene of Saccha...
We have introduced mutations into a tRNALeu3 gene which alter the intron boundaries and examined the...
AbstracttRNA splicing is a fundamental process required for cell growth and division. The first step...
Pre-mRNA splicing is the process by which intervening sequences (introns) are removed from the codin...
Introns are encoded in the genes for tRNA in organisms from all three kingdoms of life. Their remova...
AbstractThe splicing of tRNA precursors is essential for the production of mature tRNA in organisms ...
Most eukaryotic genes contain introns that interrupt the continuity of the genetic information. Intr...
My project employing the yeast model organism, Saccharomyces cerevisiae, seeks to identify gene prod...
Introns interrupt the continuity of many eukaryal genes, and therefore their removal by splicing is ...
AbstractThe rna82 mutation of Saccharomyces cerevisiae inactivates an RNA processing activity respon...
Aminoacyl tRNA synthetases (aaRS) are enzymes that add particular amino acids to cognate tRNAs. Thes...
The process of gene splicing, which involves the excision of introns from a pre-mRNA and joining of ...
AbstractStrains of Saccharomyces cerevisiae have been constructed that possess temperature-sensitive...
The Saccharomyces cerevisiae amber suppressor tRNA gene SUP53 (a tRNALeu3 allele) was used to invest...
The Saccharomyces cerevisiae leucine-inserting amber suppressor tRNA gene SUP53 (a tRNALeu3 allele) ...
In vitro mutagenesis was used to create putative opal suppressor alleles of a tRNATrp gene of Saccha...
We have introduced mutations into a tRNALeu3 gene which alter the intron boundaries and examined the...
AbstracttRNA splicing is a fundamental process required for cell growth and division. The first step...
Pre-mRNA splicing is the process by which intervening sequences (introns) are removed from the codin...
Introns are encoded in the genes for tRNA in organisms from all three kingdoms of life. Their remova...
AbstractThe splicing of tRNA precursors is essential for the production of mature tRNA in organisms ...
Most eukaryotic genes contain introns that interrupt the continuity of the genetic information. Intr...
My project employing the yeast model organism, Saccharomyces cerevisiae, seeks to identify gene prod...
Introns interrupt the continuity of many eukaryal genes, and therefore their removal by splicing is ...
AbstractThe rna82 mutation of Saccharomyces cerevisiae inactivates an RNA processing activity respon...
Aminoacyl tRNA synthetases (aaRS) are enzymes that add particular amino acids to cognate tRNAs. Thes...
The process of gene splicing, which involves the excision of introns from a pre-mRNA and joining of ...
AbstractStrains of Saccharomyces cerevisiae have been constructed that possess temperature-sensitive...