Add to ORKGCellular proteins that bear reactive azides can be imaged by fluorescence microscopy following strain-promoted ligation to cyclooctyne dyes. Here we describe BODIPY-cyclooctyne (BDPY), a membrane-permeant fluorophore that can be used to label intracellular proteins in live mammalian cells. Flow cytometry reveals fluorescence signals more than 25-fold above background after labeling of azide-tagged cells with BDPY
Twenty-five years ago, GFP revolutionized the field of cell biology by enabling scientists to visua...
Individual proteins chemically labeled with fluorescent dyes can be localized and tracked in real-ti...
The fluorescent tagging of proteins in the natural environment of the cell is an emerging technique ...
Live and let dye: Three coumarin-cyclooctyne conjugates have been used to label proteins tagged with...
The proteome undergoes complex changes in response to disease, drug treatment, and normal cellular s...
A multi-color labelling technique for visualizing multiple intracellular apparatuses in their native...
Modern proteomic methods enable efficient identification of the hundreds or thousands of proteins pr...
Over the past years, fluorescent proteins (e.g., green fluorescent proteins) have been widely utiliz...
Over the past years, fluorescent proteins (e.g., green fluorescent proteins) have been widely utiliz...
Fluorescence labelling of an intracellular biomolecule in native living cells is a powerful strategy...
Fluorescence labelling of an intracellular biomolecule in native living cells is a powerful strategy...
We report on the first, to our knowledge, successful detection of a fluorescent unnatural amino acid...
There is much recent interest in the application of copper-free click chemistry to study a wide rang...
The development of molecular probes to visualize cellular processes is an important challenge in che...
Methods that enable the super-resolution imaging of intracellular proteins in live bacterial cells p...
Twenty-five years ago, GFP revolutionized the field of cell biology by enabling scientists to visua...
Individual proteins chemically labeled with fluorescent dyes can be localized and tracked in real-ti...
The fluorescent tagging of proteins in the natural environment of the cell is an emerging technique ...
Live and let dye: Three coumarin-cyclooctyne conjugates have been used to label proteins tagged with...
The proteome undergoes complex changes in response to disease, drug treatment, and normal cellular s...
A multi-color labelling technique for visualizing multiple intracellular apparatuses in their native...
Modern proteomic methods enable efficient identification of the hundreds or thousands of proteins pr...
Over the past years, fluorescent proteins (e.g., green fluorescent proteins) have been widely utiliz...
Over the past years, fluorescent proteins (e.g., green fluorescent proteins) have been widely utiliz...
Fluorescence labelling of an intracellular biomolecule in native living cells is a powerful strategy...
Fluorescence labelling of an intracellular biomolecule in native living cells is a powerful strategy...
We report on the first, to our knowledge, successful detection of a fluorescent unnatural amino acid...
There is much recent interest in the application of copper-free click chemistry to study a wide rang...
The development of molecular probes to visualize cellular processes is an important challenge in che...
Methods that enable the super-resolution imaging of intracellular proteins in live bacterial cells p...
Twenty-five years ago, GFP revolutionized the field of cell biology by enabling scientists to visua...
Individual proteins chemically labeled with fluorescent dyes can be localized and tracked in real-ti...
The fluorescent tagging of proteins in the natural environment of the cell is an emerging technique ...