beta-1,4-Galactosyltransferase 1 (Gal-T1) transfers galactose (Gal) from UDP-Gal to N-acetylglucosamine (GlcNAc), which constitutes its normal galactosyltransferase (Gal-T) activity. In the presence of alpha -lactalbumin (LA), it transfers Gal to Glc, which is its lactose synthase (LS) activity. It also transfers glucose (Glc) from UDP-Glc to GlcNAc, constituting the glucosyltransferase (Glc-T) activity, albeit at an efficiency of only 0.3-0.4% of Gal-T activity. In the present study, we show that LA increases this activity almost 30-fold. It also enhances the Glc-T activity toward various N-acyl substituted glucosamine acceptors. Steady state kinetic studies of Glc-T reaction show that the Km for the donor and acceptor substrates are high ...
AbstractWe have isolated from bovine colostrum the lactose analog GalNAcβ1→4Glc. The enzymatic basis...
Utilising a fast and sensitive screening method based on imidazolium-tagged probes, we report unprec...
UDP-GalNAc: polypeptide N-acetylgalactosaminyltransferases (GalNAc-transferases) catalyze an initia...
The capacity of UDP-N-acetyl-α-D-glucosamine (UDP-GlcNAc) as an in vitro acceptor substrate for β-1,...
Lipopolysaccharyl-alpha-1,4-galactosyltransferase C (LgtC), a glycosyltransferase family 8 alpha-1,4...
AbstractIn order to assess the function of the different human UDP-Gal:GlcNAc β4-galactosyltransfera...
The mutant β1,4-galactosyltransferase (β4Gal-T1), β4Gal-T1-Y289L, in contrast to wild-type β4Gal-T1,...
alpha-Lactalbumin (alpha-LA) is a regulatory protein by which the mammalian beta1,4-galactosyltransf...
Through the use of affinity chromatography, a homogeneous preparation of human β(1→4) D glactosyltra...
Glycosyltransferases (GTs), the enzymes that catalyse glycosidic bond formation, create a diverse ra...
The initial step of mucin-type O-glycosylation is catalyzed by UDP-GalNAc : polypeptide N-acetylgal...
Pyridylamino asialo-agalacto-biantennary sugar chain (PA-acceptor), prepared from human a,-acid glyc...
Human O-GlcNAcase plays an important role in regulating the post-translational modification of ...
AbstractGalactokinase (GALK), a member the Leloir pathway for normal galactose metabolism, catalyzes...
Lipopolysaccharyl α-1,4-galactosyltransferase C (EC 2.4.1.x, LgtC) from Neisseria meningitidis is a...
AbstractWe have isolated from bovine colostrum the lactose analog GalNAcβ1→4Glc. The enzymatic basis...
Utilising a fast and sensitive screening method based on imidazolium-tagged probes, we report unprec...
UDP-GalNAc: polypeptide N-acetylgalactosaminyltransferases (GalNAc-transferases) catalyze an initia...
The capacity of UDP-N-acetyl-α-D-glucosamine (UDP-GlcNAc) as an in vitro acceptor substrate for β-1,...
Lipopolysaccharyl-alpha-1,4-galactosyltransferase C (LgtC), a glycosyltransferase family 8 alpha-1,4...
AbstractIn order to assess the function of the different human UDP-Gal:GlcNAc β4-galactosyltransfera...
The mutant β1,4-galactosyltransferase (β4Gal-T1), β4Gal-T1-Y289L, in contrast to wild-type β4Gal-T1,...
alpha-Lactalbumin (alpha-LA) is a regulatory protein by which the mammalian beta1,4-galactosyltransf...
Through the use of affinity chromatography, a homogeneous preparation of human β(1→4) D glactosyltra...
Glycosyltransferases (GTs), the enzymes that catalyse glycosidic bond formation, create a diverse ra...
The initial step of mucin-type O-glycosylation is catalyzed by UDP-GalNAc : polypeptide N-acetylgal...
Pyridylamino asialo-agalacto-biantennary sugar chain (PA-acceptor), prepared from human a,-acid glyc...
Human O-GlcNAcase plays an important role in regulating the post-translational modification of ...
AbstractGalactokinase (GALK), a member the Leloir pathway for normal galactose metabolism, catalyzes...
Lipopolysaccharyl α-1,4-galactosyltransferase C (EC 2.4.1.x, LgtC) from Neisseria meningitidis is a...
AbstractWe have isolated from bovine colostrum the lactose analog GalNAcβ1→4Glc. The enzymatic basis...
Utilising a fast and sensitive screening method based on imidazolium-tagged probes, we report unprec...
UDP-GalNAc: polypeptide N-acetylgalactosaminyltransferases (GalNAc-transferases) catalyze an initia...