Random Amplified Polymorphic Dna (Rapd) Profiles of Several Isolates of Ganoderma Spp. From Banyumas, Central Java, Indonesia

A study on the RAPD profiles of several isolates of Ganoderma spp. from Banyumas Regency, Central Java, Indonesia and their genetic inter-relationship based on RAPD markers was conducted using a survey method, where random sampling technique was applied. Collected Ganoderma spp. fruiting bodies were first morphologically characterized, after which RAPD analysis utilizing four random primers, i.e. OPC-1, OPC-2, OPC-4 and OPC-5 was carried out. The results revealed that the four primers used generated polymorphic bands toward 10 samples with polymorphism level of 100%, showing absolutely high genetic diversity. The level of genetic similarity ranged between 0.48 and 0.82, indicating moderate similarities among samples. A constructed dendogram indicated that the isolates of Ganoderma spp. were grouped into three clusters at a coefficient of 0.63, but neither according to geographical locations nor growth substrates