Add to ORKGThe theory for measuring ligand binding constants from footprinting autoradiographic data associated with a single binding site is derived. If the ligand and DNA cleavage agent compete for a common site, the spot intensities are not proportional to the amount of DNA not blocked by ligand. The analysis of a single site is experimentally illustrated by using results for the anticancer drug actinomycin D interacting with the duplex d(TAGCGCTA)2 as probed with the hydrolytic enzyme DNase I
This chapter describes three complementary footprinting methods and the protocols used for analysis ...
AbstractWe have used a modification of the footprinting technique to measure the dissociation of mit...
Sequence-dependent thermodynamic quantities for the antiviral agent netropsin and a related bis(N-me...
The results of quantitative footprinting studies involving the antiviral agent netropsin and a DNA-c...
A theory for deriving drug-DNA site binding constants from footprinting data is presented. Plots of ...
In footprinting experiments, an increase in DNA cleavage with addition of ligand to a system may be ...
Footprinting is a simple method for assessing the sequence selectivity of DNA-binding ligands. The m...
We report site-specific binding constants for the intercalating anticancer drug actinomycin D (Act-D...
The established protocol for DNase I footprinting has been modified to allow multiple parallel react...
A high-throughput Cognate Site Identity (CSI) microarray platform interrogating all 524 800 10-base ...
Footprinting experiments for DNase I digests of a 139-base-pair segment of pBR-322 DNA in the presen...
Chromomycin A3 (CHR) binding to the duplex d(CAAGTCTGGCCATCAGTC)- d(GACTGATGGCCAGACTTG) has been stu...
Part of the Chemistry Commons This Article is brought to you for free and open access by the College...
Footprinting is the process of identifying the specific sites on DNA to which a molecule, usually a ...
DNase I and MPE.Fe (II) footprinting both employ partial cleavage of ligand-protected DNA restrictio...
This chapter describes three complementary footprinting methods and the protocols used for analysis ...
AbstractWe have used a modification of the footprinting technique to measure the dissociation of mit...
Sequence-dependent thermodynamic quantities for the antiviral agent netropsin and a related bis(N-me...
The results of quantitative footprinting studies involving the antiviral agent netropsin and a DNA-c...
A theory for deriving drug-DNA site binding constants from footprinting data is presented. Plots of ...
In footprinting experiments, an increase in DNA cleavage with addition of ligand to a system may be ...
Footprinting is a simple method for assessing the sequence selectivity of DNA-binding ligands. The m...
We report site-specific binding constants for the intercalating anticancer drug actinomycin D (Act-D...
The established protocol for DNase I footprinting has been modified to allow multiple parallel react...
A high-throughput Cognate Site Identity (CSI) microarray platform interrogating all 524 800 10-base ...
Footprinting experiments for DNase I digests of a 139-base-pair segment of pBR-322 DNA in the presen...
Chromomycin A3 (CHR) binding to the duplex d(CAAGTCTGGCCATCAGTC)- d(GACTGATGGCCAGACTTG) has been stu...
Part of the Chemistry Commons This Article is brought to you for free and open access by the College...
Footprinting is the process of identifying the specific sites on DNA to which a molecule, usually a ...
DNase I and MPE.Fe (II) footprinting both employ partial cleavage of ligand-protected DNA restrictio...
This chapter describes three complementary footprinting methods and the protocols used for analysis ...
AbstractWe have used a modification of the footprinting technique to measure the dissociation of mit...
Sequence-dependent thermodynamic quantities for the antiviral agent netropsin and a related bis(N-me...