Partial Purification and Characterization of Antigonadotropic Activity of Bovine Pineal Glands

This study is concerned with the purification and characterization of an antigonaclotropin from bovine pineal glands.. The biological assay is based on the compensating increase in rate of growth of the intact ovary after removal of the one ovary. The inhibition of such compensatory growth was assumed to be due to the antigonadotropin. All purification steps were. based on the assumption that the antigonadotropin has peptide characteristics. Thus, each purification step was monitored by the standard absorption wave-lengths of 280 nm and 220 nm.Biochemistr