Verfeinerte parapation biologischer Gewebeproben für raster Elektronmikroskopie.

A simple and convenient method is described for sticking biological tissue samples onto a small copper net using Histoacryl (the n butyl ester out of the series of α cyanoacrylic acid esters). The technique has the following advantages: Wet samples can be quickly stuck to the net, and are thus protected while being handled further in different baths. The use of copper facilitates the process of freeze drying effectively, and the net structure of the holder stimultaneously allows drying of the entire sample surface. Thus, in most cases the cumbersome and expensive preparation of delicate mechanical sample holders can be circumvented