<em>Chryseobacterium hispalense</em> sp. nov., a plant growth-promoting bacterium isolated from a rainwater pond in an olive plant nursery and emendation of the species <em>Chryseobacterium defluvii</em>, <em>Chryseobacterium indologenes</em>, <em>Chryseo

A novel non-motile, Gram-staining-negative, yellow-pigmented bacterium, designated AG13T, isolated from a rain water pond in a plant nursery in Spain and characterised as a plant growth promoting bacterium, was investigated to determine its taxonomic status. The isolate grew best over a temperature range of 15-40&deg;C and at pH 5.0-8.0 and with 0-4% (w/v) NaCl. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Chryseobacterium. The DNA G + C content of the novel strain was 37.2 mol%. The strain contained a polyamine pattern with sym-homospermidine as the major compound and produced flexirubin-type pigments. MK-6 was the dominant menaquinone and the major cellular fatty acids were iso-C15:0, C17:1&omega;9c and iso-C17:0 3-OH. The main polar lipids were phosphatidylethanolamine, aminolipids and several unidentified lipids. The 16S rRNA gene showed 92.0-97.2% sequence identity with the members of the genus Chyseobacterium. Based on chemotaxonomic, phenotypic traits and DNA-DNA hybridizations with the type strains of the most closely related species, the isolate is proposed to represent a novel species, Chyseobacterium hispalense, type strain AG13T (= DSM 25574T = CCUG 63019T). Emended descriptions of the species Chryseobacterium defluvii, Chryseobacterium indologenes, Chryseobacterium wanjuense and Chryseobacterium gregarium are also provided