Background Quantitation of antigen-specific T cells provides an insight into the development and dynamics of T-cell responses in tumor immunology and infectious diseases. Soluble major histocompatibility class I tetramers are widely used to monitor immune responses; however, variations due to handling and analysis are likely to confound comparisons between different experiments and laboratories. Methods Whole blood from healthy donors was stained with HLA-A*0201/tetramers specific for an epitope of phosphoprotein 65, the immunodominant antigen in cytomegalovirus infection. With the help of Trucount tubes, a single-platform, four-color flow cytometric assay was established to obtain absolute counts of tetramer-positiv...
International audienceBackground: Quantification of regulatory T cells (Tregs) is crucial in immunom...
Enumeration of anti-viral CD8+ T cells to make comparisons between mice, viruses and vaccines is a f...
Peptide-bound histocompatibility leukocyte antigen (HLA) class I tetramers enable a precise identifi...
Major histocompatibility complex (MHC) multimers that identify antigen- specific T cells, coupled wi...
BACKGROUND: HLA class I peptide tetramers represent powerful diagnostic tools for detection and moni...
With the advent of novel strategies to induce tolerance in autoimmune and autoimmune-like conditions...
With the advent of novel strategies to induce tolerance in autoimmune and autoimmune-like conditions...
With the advent of novel strategies to induce tolerance in autoimmune and autoimmune-like conditions...
The development of the fluorescently labeled tetrameric MHC-peptide complex has enabled the direct v...
The direct detection of antigen-specific T cells using tetramers of soluble peptide-major histocompa...
<p>The gating hierarchy and tetramer positive cells in a patient expressing 3 of the HLA molecules r...
MHC class I-peptide tetrameric complexes ('tetramers') have revolutionized the study of antiviral CD...
The development of peptide–MHC tetrameric complexes heralds a new era in the study of antigen-specif...
Fluorochrome-conjugated peptide-major histocompatibility complex (pMHC) multimers are widely used fo...
The ability to quantify and characterize antigen-specific CD8+ T cells irrespective of functional re...
International audienceBackground: Quantification of regulatory T cells (Tregs) is crucial in immunom...
Enumeration of anti-viral CD8+ T cells to make comparisons between mice, viruses and vaccines is a f...
Peptide-bound histocompatibility leukocyte antigen (HLA) class I tetramers enable a precise identifi...
Major histocompatibility complex (MHC) multimers that identify antigen- specific T cells, coupled wi...
BACKGROUND: HLA class I peptide tetramers represent powerful diagnostic tools for detection and moni...
With the advent of novel strategies to induce tolerance in autoimmune and autoimmune-like conditions...
With the advent of novel strategies to induce tolerance in autoimmune and autoimmune-like conditions...
With the advent of novel strategies to induce tolerance in autoimmune and autoimmune-like conditions...
The development of the fluorescently labeled tetrameric MHC-peptide complex has enabled the direct v...
The direct detection of antigen-specific T cells using tetramers of soluble peptide-major histocompa...
<p>The gating hierarchy and tetramer positive cells in a patient expressing 3 of the HLA molecules r...
MHC class I-peptide tetrameric complexes ('tetramers') have revolutionized the study of antiviral CD...
The development of peptide–MHC tetrameric complexes heralds a new era in the study of antigen-specif...
Fluorochrome-conjugated peptide-major histocompatibility complex (pMHC) multimers are widely used fo...
The ability to quantify and characterize antigen-specific CD8+ T cells irrespective of functional re...
International audienceBackground: Quantification of regulatory T cells (Tregs) is crucial in immunom...
Enumeration of anti-viral CD8+ T cells to make comparisons between mice, viruses and vaccines is a f...
Peptide-bound histocompatibility leukocyte antigen (HLA) class I tetramers enable a precise identifi...