A comparative study of most probable number (MPN)-PCR vs. real-time-PCR for the measurement of abundance and assessment of diversity of alkB homologous genes in soil.
- Schulz, S.
- Pérez-de-Mora, A.
- Engel, M.
- Munch, J.-C.
- Schloter, M.
Publication date
January 2010
DOI Publisher
Elsevier BV ISSN
0167-7012 Citation count (estimate)
19Abstract
The utilization of quantitative PCR (qPCR) approaches such as MPN-qPCR and real-time-qPCR for in situ assessment of functional genes yields substantial quantitative and qualitative differences. We show this by targeting the alkB gene related to biodegradation of aliphatic alkanes in a set of environmental samples with differing hydrocarbon content
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