The microbial ferrous wheel in a neutral pH groundwater seep

Evidence for microbial Fe redox cycling was documented in a circumneutral pH ground-water seep near Bloomington, Indiana. Geochemical and microbiological analyses were conducted at two sites, a semi-consolidated microbial mat and a floating puffball structure. In situ voltammetric microelectrode measurements revealed steep opposing gradients of o2 and Fe(I I) at both sites, similar to other groundwater seep and sedimentary environments known to support microbial Fe redox cycling. The puffball structure showed an abrupt increase in dissolved Fed I) just at its surface (~5cm depth), suggesting an internal Fe(I I) source coupled to active Fed 1I) reduction. Most probable number enumerations detected microaerophilic Fe(II)-oxidizing bacteria (FeoB) and dissimilatory Fe(III)-reducing bacteria (FeRB) at densities of 102 to 105 cells ml_~1 in samples from both sites. In vitro Fed 1I) reduction experiments revealed the potential for immediate reduction (no lag period) of native Fe(III) oxides. Conventional full-length 16S rRNA gene clone libraries were compared with high throughput barcode sequencing of theV1, V4, orV6 variable regions of 16S rRNA genes in order to evaluate the extent to which new sequencing approaches could provide enhanced insight into the composition of Fe redox cycling microbial community structure. The composition of the clone libraries suggested a lithotroph-dominated microbial community centered around taxa related to known FeoB (e.g., Gallionella, Sideroxydans, Aquabacterium). Sequences related to recognized FeRB (e.g., Rhodoferax, Aeromonas, Geobacter, Desulfovibrio) were also well-represented. overall, sequences related to known FeoB and FeRB accounted for 88 and 59% of total clone sequences in the mat and puffball libraries, respectively. Taxa identified in the barcode libraries showed partial overlap with the clone libraries, but were not always consistent across different variable regions and sequencing platforms. However, the barcode libraries provided confirmation of key clone library results (e.g., the predominance of Betaproteobacteria) and an expanded view of lithotrophic microbial community composition