Serum Lipidomics Study of Polycystic Ovarian Syndrome Based on Liquid Chromatography-Mass Spectrometry

Polycystic ovarian syndrome (PCOS) is one of the most common endocrine disorders in fertile women and contributes to female infertility. Polycystic ovarian syndrome is heterogeneous and its pathogenesis has not been clearly elucidated. In this study, sera from 74 PCOS patients and 54 healthy persons as control were analyzed based on reversed. phase high performance liquid chromatography coupled with quadrupole time. of. flight mass spectrometry (RPLC/Q-TOF-MS). A total of 314 lipid compounds of 15 classes were indentified. Non. parametric statistical test was performed on metabolomics data and p < 0.05 was considered as significant. Compared with normal women, the serum levels of unsaturated fatty acids, most sphingomyelins, diacylglycerol and triglyceridewere were elevated, while the levels of mostphosphatidylcholine, lyso. phosphatidylcholine, lyso. phosphatidylethanolamine were reduced in PCOS women. Furthermore, in obese PCOS women, the higher levels of unsaturated fatty acids, sphingomyelins, diacylglycerol and triglyceride, and the lower levels of most phosphatidylcholine and lyso. phosphatidylcholine were found. This study demonstrated that lipidomics is a powerful tool for disease classification