Endotoxin was removed by affinity membranes with histidine immobilized as affinity ligand. Macropore cellulose membrane was prepared from filter paper by alkaline treatment and chemical crosslinking, and was used as matrix for the immobilization of affinity ligand. The matrix membrane was derived by hexamethylenediamine and activated by glutaraldehyde before histidine was immobilized. Membrane cartridges containing 40 or 80 sheets of affinity membrane were also prepared, which can be used to remove pyrogen from aqueous solutions. Using a cartridge with 40 sheets of affinity membrane, the endotoxin content in solution can be reduced to a minimum of 0.12 EU/mL. (C) 1997 by John Wiley & Sons, Ltd
ABSTRACTPURPOSE: Endotoxins, also called lipopolysaccharides (LPS), are major contaminants found in ...
The production of biopharmaceutical proteins by means of Escherichia coli cultivation leads to conta...
honors thesisCollege of EngineeringBiomedical EngineeringSusan BockEndotoxins (also known as lipopol...
Endotoxin was removed by membrane affinity chromatography. Macropore cellulose membrane was prepared...
A novel matrix of macropore cellulose membrane was prepared by chemical graft, and immobilized the c...
ABSTRACT The surface of flat-sheet nylon membranes was modified using bisoxirane as the spacer and p...
Affinity sorbents and detoxification strategies are described to remove different amounts of endotox...
AbstractThe presence of endotoxins in preparations of recombinantly produced therapeutic proteins po...
The purpose of the work: receiving the endotoxines from the cells of bacteria Ps. putida (36) and E....
Three affinity matrices for adsorption of endooxin from aqueous solution were studied by covalently ...
The affinity filtration technique using histidine as the pseudobiospecific ligand immobilized on pol...
Lipopolysaccharide (LPS, endotoxin, pyrogen) constitutes a very troubling contaminant of crude phage...
Endotoxin is a lipopolysaccharide found on the membrane of gram-negative bacteria and is extremely t...
The adsorptive filtration technique using chitosan membranes was used to remove endotoxin (ET) from ...
Lipopolysaccharide (LPS, endotoxin, pyrogen) constitutes a very troubling contaminant of crude phage...
ABSTRACTPURPOSE: Endotoxins, also called lipopolysaccharides (LPS), are major contaminants found in ...
The production of biopharmaceutical proteins by means of Escherichia coli cultivation leads to conta...
honors thesisCollege of EngineeringBiomedical EngineeringSusan BockEndotoxins (also known as lipopol...
Endotoxin was removed by membrane affinity chromatography. Macropore cellulose membrane was prepared...
A novel matrix of macropore cellulose membrane was prepared by chemical graft, and immobilized the c...
ABSTRACT The surface of flat-sheet nylon membranes was modified using bisoxirane as the spacer and p...
Affinity sorbents and detoxification strategies are described to remove different amounts of endotox...
AbstractThe presence of endotoxins in preparations of recombinantly produced therapeutic proteins po...
The purpose of the work: receiving the endotoxines from the cells of bacteria Ps. putida (36) and E....
Three affinity matrices for adsorption of endooxin from aqueous solution were studied by covalently ...
The affinity filtration technique using histidine as the pseudobiospecific ligand immobilized on pol...
Lipopolysaccharide (LPS, endotoxin, pyrogen) constitutes a very troubling contaminant of crude phage...
Endotoxin is a lipopolysaccharide found on the membrane of gram-negative bacteria and is extremely t...
The adsorptive filtration technique using chitosan membranes was used to remove endotoxin (ET) from ...
Lipopolysaccharide (LPS, endotoxin, pyrogen) constitutes a very troubling contaminant of crude phage...
ABSTRACTPURPOSE: Endotoxins, also called lipopolysaccharides (LPS), are major contaminants found in ...
The production of biopharmaceutical proteins by means of Escherichia coli cultivation leads to conta...
honors thesisCollege of EngineeringBiomedical EngineeringSusan BockEndotoxins (also known as lipopol...