Identification of Peptide Profiling of Deer Antler by Reversed Phase Liquid Chromatography-Tandem Mass Spectrometry and Bioactivities in Simulated Gastrointestinal Digestion

Reversed phase liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) was utilized to investigate peptide profiling and bioactivities of antler aqueous extract (AAE), digested antler aqueous extract (AED) and powder (PD). A total of 23, 417 and 389 peptides, as well as 15, 146 and 75 collagen peptides were identified from AAE, AED and PD, respectively. Angiotensin converting enzyme (ACE) inhibitory activity, dipeptidyl peptidase IV (DPP-IV) inhibitory activity, prolyl endopeptidase (PEP) inhibitory activity and antioxidant activity were used to evaluate the bioactivities of AAE, AED and PD, and it was found that the sequence of their bioactivities was AAE < AED < PD. All the results above proved that AED released more collagen peptides and PD possessed better biological activities. It suggests that the two edible ways of antler are complemented each other and have their own advantages