Cholesteatoma - the Neoangiogenesis in Adults and Children

Оbjective: The aim of the current study was: to detect the presence of large and newly form small blood vessels in chronic inflammation of cholesteatoma and to evaluate the role of VEGF and CD-31 in triggering of angiogenesis in chronic inflammation of cholesteatoma in adults and childrenMaterial & Methods: The CD-31- positive blood vessels and the level of expression of VEGF were determined by immunohistochemistry 16 archival biopsies which from patients with adults cholesteatoma and 5 patients with pediatric cholesteatomaResults: CD31-positive blood vessels in the granulation tissue of cholesteatoma were more numerous than the vessels in the adjacent normal skin of the auditory meatus; VEGF-positive blood vessels in the granulation tissue cholesteatoma are more numerous that the vessels in the adjacent normal skin of the auditory meatus.Conclusion: The observed increased angiogenesis in granulation tissue of cholesteatoma might be triggered by the increased expression of VEGF which is secreted by many structures and cells in perimatrix of cholesteatoma. The studies characteristics of the active angiogenesis were markedly enhanced in the biopsies from congenital cholesteatoma form. The results suggest the important role of angiogenesis in the growth of cholesteatoma and in aggressiveness of congenital pediatric typeKey words: Cholesteatoma, Adults, Children, Angiogenesis, VEGF, CD-3