Effect of Sample Injection Volume on Band Broadening in High Performance Liquid Chromatography

Sample injection volume is one of the separation variables in liquid chromatography and it affects resolution. To investigate the effect of sample injection volume on band broadening, we made injections of various volumes of a given eluite while keeping the injection amount constant, and these studies comprise the present thesis. The Introduction of this thesis states what currently has been achieved on this subject by other workers and provides background information. The following two sections describe how the data were obtained. The Results and Discussion section presents the reversed-phase high performance liquid chromatography data obtained along with some regression analyses. It is hoped that this investigation will improve our understanding of the mechanism of the involved processes