The quantitative analysis of thiamin and riboflavin and their respective vitamers in fermented alcoholic beverages

This research aimed to develop a simple and effective method for analyzing thiamin (B 1), riboflavin (B 2) and their respective vitamers by high performance liquid chromatography (HPLC) in fermented alcoholic beverages. The method developed here employs a phosphate buffer/methanol gradient elution on a single reverse phase column, coupled with independent fluorescent detection regimes. It also employs a precolumn derivatization to convert thiamin to thiochrome via an alkaline potassium ferricyanide solution. The method described here allowed a spike recovery of better than 97%, with a typical linear detection range (R 2 ≥ 0.9997) between ≥5 and ≥50