Caenorhabditis elegans: The Search for srf-6 Mutations

Parasitic nematodes may evade host immune systems by changing surface composition during infection. C. elegans is a free-living nematode with a conserved development and cuticle structure utilized as a parasitic model. srf-6 mutant worms fail to make surface antigen switches that occur facultatively in the wild type. The whole genome sequences of three srf-6 mutants were determined to identify the DNA sequence corresponding to srf-6. This project’s objective was to find and confirm srf-6 mutations in the genome sequence by re-sequencing the candidate gene in mutant DNA