3D Electrospun Tissue Scaffold: Achieving Customized Fiber Alignment

Engineering of 3D scaffolds for tissue repair requires the ability to align cells to mimic cell orientations of native tissues. Our study focused on utilizing electrospinning technology to develop 3D nanofiber scaffolds whose alignment can be customized to mimic the microarchitecture of a tissue. Current electrospinning techniques have limited control of fiber orientation. We engineered a collection plate that can be adapted to produce 3D biomaterial scaffolds for use with commercially available electrospinning units. The custom fiber aligned scaffolds were seeded with green fluorescent protein (GFP) expressing cells to show viability for cell seeding and alignment along the fiber direction. The major device design characteristics and results are presented in this report