Investigation of the Propensity for Self-Association of the N-Terminal Domain of Annexin A2 in the Presence of Anionic Lipids

Annexin A2 (ANXA2), a calcium-dependent membrane binding protein shown to promote membrane domain formation, is implicated in many cellular processes. In order to determine if ANXA2 promotes domain formation by self-associating with the N-terminal domain of adjacent ANXA2 proteins upon binding anionic phospholipids, Forster Resonance Energy Transfer (FRET) assays were performed using ANXA2 proteins fluorescently labeled at the singular, exposed N-terminal cysteine residue. No FRET transfer was observed, suggesting that under these experimental conditions the N-terminal domains do not interact