CBF Regulation on RAG Genes Via Erag Enhancer

This project explores the function of Core Binding Factor (CBF) in B-cell development, and its regulation on the recombination activating genes (RAG) that encode the B-cell antibody gene recombinase via Erag enhancer. A GFP reporter construct containing Erag and the RAG2 promoter was transfected into pro-B cells, and was mutated to block CBF binding. Leukemia cells and transfected pro-B cells were treated with CBF inhibitor to measure the effects of disrupted CBF function. The transcript levels of B-cell specific proteins of inhibitor-treated cells were measured by qPCR. The data indicate cell viability and key B cell protein expressions were decreased, but reporter activity was not decreased after inhibitor treatment. The mutant was obtained but was not yet transfected