Production, purification and partial characterization of a novel protease from marine Engyodontium album BTMFS10 under solid state fermentation

Chandrasekaran, M
Sreeja, Chellappan
Jasmin, C
Soorej, Basheer M
Elyas, K K
Sarita,G Bhat

Publication date

October 2005

Publisher

Elsevier BV

Abstract

Engyodontium album isolated from marine sediment produced protease, which was active at pH 11. Process parameters influencing the production of alkaline protease by marine E. album was optimized. Particle size of <425 mm, 60% initial moisture content and incubation at 25 8C for 120 h were optimal for protease production under solid state fermentation (SSF) using wheat bran. The organism has two optimal pH (5 and 10) for maximal enzyme production. Sucrose as carbon source, ammonium hydrogen carbonate as additional inorganic nitrogen source and amino acid leucine enhanced enzyme production during SSF. The protease was purified and partially characterized. A 16-fold purified enzyme was obtained after ammonium sulphate precipitation and ion-exc...

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Production, purification and partial characterization of a novel protease from marine Engyodontium album BTMFS10 under solid state fermentation

Abstract

Extracted data

Production, purification and partial characterization of a novel protease from marine Engyodontium album BTMFS10 under solid state fermentation

Abstract

Extracted data

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