tWe evaluated at-column dilution (ACD) as a possible approach to reduce peak distortion caused by theinjection of strong solvents in preparative hydrophilic interaction chromatography (HILIC). Three modelcompounds, namely uridine, cytidine and guanosine were selected and injected either in conventional orACD conditions, using various proportions of water and acetonitrile in the sample diluent. Plate numberand peak capacity were systematically investigated under both isocratic and gradient elution conditions,respectively. In isocratic conditions, ACD was found to provide higher plate numbers with sample diluentcontaining more than 40% water, but lower plate numbers with injections of less than 40% water, incomparison to a conventional inject...
The separation of compounds possessing low solubility in the mobile phase could be improved by apply...
This article is an update on the technique of hydrophilic interaction liquid chromatography (HILIC) ...
For a wide variety of hydrophilic interaction chromatography stationary phases, a repeatable partial...
Sample injection can cause serious problems in hydrophilic interaction liquid chromatography (HILIC)...
The aim of this study was to evaluate the importance of sample diluents to improve peak shapes in hy...
To be productive the columns are usually overloaded in preparative chromatography. It is well known ...
The separation of acidic, neutral and particularly basic solutes was investigated using a bare silic...
© 2015 Elsevier B.V. Some factors which present difficulties for obtaining good peak shape in hydrop...
In preparative chromatography often the solubility of samples to be separated is low in mobile phase...
Hydrophilic interaction liquid chromatography (HILIC) has grown in popularity during the last ten ye...
Chromatography is, in principle, a dilution process. In HPLC analysis, on dis-solving the substances...
In the present study, the possibility of retention modeling in the HILIC mode was investigated, test...
The retention and peak shape of neutral, basic and acidic solutes was studied on hydrophilic interac...
This work is meant as a contribution to increase the understanding of preparative batch liquid chrom...
The sample capacity, column efficiency (and its variation with flow) of a superficially porous unbon...
The separation of compounds possessing low solubility in the mobile phase could be improved by apply...
This article is an update on the technique of hydrophilic interaction liquid chromatography (HILIC) ...
For a wide variety of hydrophilic interaction chromatography stationary phases, a repeatable partial...
Sample injection can cause serious problems in hydrophilic interaction liquid chromatography (HILIC)...
The aim of this study was to evaluate the importance of sample diluents to improve peak shapes in hy...
To be productive the columns are usually overloaded in preparative chromatography. It is well known ...
The separation of acidic, neutral and particularly basic solutes was investigated using a bare silic...
© 2015 Elsevier B.V. Some factors which present difficulties for obtaining good peak shape in hydrop...
In preparative chromatography often the solubility of samples to be separated is low in mobile phase...
Hydrophilic interaction liquid chromatography (HILIC) has grown in popularity during the last ten ye...
Chromatography is, in principle, a dilution process. In HPLC analysis, on dis-solving the substances...
In the present study, the possibility of retention modeling in the HILIC mode was investigated, test...
The retention and peak shape of neutral, basic and acidic solutes was studied on hydrophilic interac...
This work is meant as a contribution to increase the understanding of preparative batch liquid chrom...
The sample capacity, column efficiency (and its variation with flow) of a superficially porous unbon...
The separation of compounds possessing low solubility in the mobile phase could be improved by apply...
This article is an update on the technique of hydrophilic interaction liquid chromatography (HILIC) ...
For a wide variety of hydrophilic interaction chromatography stationary phases, a repeatable partial...