Estudo da atividade antioxidante e antitumoral de biocompostos de macromicetos produzidos em fermentação submersa em co-cultivo

Orientador : Prof. Dr. Carlos Ricardo SoccolCo-orientador : Prof. Dr. Miguel NosedaTese (doutorado) - Universidade Federal do Paraná, Setor de Tecnologia, Programa de Pós-Graduaçao em Processos Biotecnológicos. Defesa: Curitiba, 11/06/2010Inclui referênciasÁrea de concentração: Biotecnologia agroalimentarAbstract: The antioxidant potential of mushrooms such as Agaricus brasiliensis, Cordyceps sinensis, Ganoderma lucidum and Grifola frondosa cultivated separately and in combination by submerged fermentation was investigated. The co-culture these species resulted in six combinations: A. brasiliensis + C. sinensis, A. brasiliensis + G. lucidum, A. brasiliensis + G. frondosa, C. sinensis + G. lucidum, C. sinensis + G. frondosa, . lucidum + G. frondosa. The submerged cultivation of mushrooms resulted in three biocompounds: ethanolic biomass extracts (EBE), fermented broth (BF) and exopolysaccharide (EPS). These compounds were evaluated for their antioxidant activity in different mechanisms: free radical scavenging capacity, inhibition of lipid peroxidation, reducing power, anion superoxide scavenging activity and total phenol compounds. DPPH capacity of biomass from G. lucidum + G. frondosa showed radical affect 79.742% } 1.72 and isolate culture of G. lucidum 79.097% } 3.629. These results are similar. The best result for the inhibition of lipid peroxidation s-carotene + linoleic acid systems was obtained with the ethanolic biomass extracts of A. brasiliensis + C. sinensis was 28.63% } 5.62 was the better results for inhibition of lipid peroxidation scarotene+ linoleic acid systems, but it is lower compared to controls. Reducing power activities showed results close to controls in most samples. A. brasiliensis + G. lucidum presented 127.58% } 2.4 for superoxide anion and 168.84 } 10.29 ƒÊg/mL Gallic Acid equivalent for quantify phenol compounds. The synergic submerged fermentation of mushrooms is alternative for obtaining natural compounds with antioxidant activity and similar effect to traditional substances