Toxicity Determination of Xenobiotics in Water

Cilj ovog istraživanja bio je odrediti toksičnost aktivnih supstancija navedenih na popisu praćenja za tvari za koje je potrebno praćenje diljem Unije u području vodne politike u skladu s europskom Direktivom 2008/105/EZ. To su makrolidni antibiotici, herbicidi, insekticidi, antioksidansi, nesteroidni antireumatici, estrogenski hormoni i sredstva za zaštitu od sunca. Primijenjena je brza i jednostavna bioanalitička metoda određivanja akutne ekotoksičnosti ksenobiotika u vodi bakterijom Vibrio fischeri. Navedena bakterija osjetljiva je na organska onečišćenja, odnosno dolazi do inhibicije rasta bakterije, što se očituje smanjenjem njezine prirodno urođene luminiscencije. U tim eksperimentima mjerila se inhibicija luminiscencije bakterije dodatkom različitih koncentracija otopina ksenobiotika. Mjerenja su pokazala kako svi ksenobiotici, osim 2-etil-heksil-6-metoksicinamata uzrokuju inhibiciju bakterijske kulture, a najtoksičniji je insekticid metiokarb. Ovo djelo je dano na korištenje pod licencom Creative Commons Imenovanje 4.0 međunarodna.The aim of this research was to determine the toxicity of the active substances listed in the watch lists for substances that need monitoring across the EU in the field of water policy according to European Directive 2008/105/EC. Those are macrolide antibiotics, herbicides, insecticides, antioxidants, nonsteroidal antireumatics, estrogen hormones, and sunscreening agents. A quick and simple bioanalytic method was used to determine the acute ecotoxicity of xenobiotics in water by the Vibrio fischeri bacteria. These bacteria are susceptible to organic contamination, bacterial growth is inhibited, which is manifested by a reduction in their inborn luminescence. In these experiments, inhibition of bacterial luminescence was measured by adding different concentrations of xenobiotic solutions. Measurements have shown that all xenobiotics, except 2-ethylhexyl-6-methoxycinnamate, cause bacterial culture inhibition, and the most toxic xenobiotic is an insecticide, methiocarb. This work is licensed under a Creative Commons Attribution 4.0 International License