Presence of biogenic amines in sparkling wine influenced by the immobilized yeast cells technology

Novi trendovi na području sigurnosti hrane povećali su fokus na elemente i spojeve u tragovima koji mogu utjecati na ljudsko zdravlje, u koje se ubrajaju i biogeni amini. Biogeni amini su biološki aktivni dušični spojevi male molekulske mase. U vinu mogu nastati iz prekursora, djelovanjem mikroorganizama u bilo kojoj fazi proizvodnje. Cilj ovoga rada bio je ispitati utjecaj fermentacije imobiliziranim kvascima na sadržaj biogenih amina u pjenušcu. Kvantitativno određivanje provedeno je pomoću RP-HPLC (Reversed Phase High Performance Liquid Chromatography) te je utvrđeno da je pjenušac proizveden pomoću imobiliziranih kvasaca sadržavao niži udio biogenih amina (5,41 mgL-1 u odnosu na 7,24 mgL-1 u klasično proizvedenom).Recent trends in food safety are promoting an increasing search for trace compounds that can affect human health, biogenic amines belong to this group of substances. Biogenic amines are basic nitrogenous low molecular weight compounds with biological activity. Biogenic amines in the wine can be formed from their precursors by various microorganisms present in the wine, at any stage of production. The aim of the present work was to investigate the changes of the content of biogenic amines in sparkling wines produced by fermentation method with immobilized yeast cells. Biogenic amines were quantified using a reversedphase high performance liquid chromatography (HPLC). Total amount of biogenic amines was 5.41 mgL-1 in wines made with immobilized yeast cells and 7.24 mgL-1 in wines made in classical fermentation process. From the results obtained in this study, it can be concluded that immobilized yeast cells technology can influence the formation of biogenic amines