Primary hypolactasia is the main cause of lactose intolerance in adults. It is strongly associated with the single genetic variant LCT-13910C>T, located upstream of the lactase encoding gene. Consequently, analysis of LCT-13910C>T has been recommended as a direct genetic test for the trait. The aim of our study was to develop a TaqMan probe based real-time PCR protocol for the detection of the LCT-13910C>T variant directly from whole blood, circumventing DNA isolation. The LCT-13910C>T variant was determined using the DirectBlood Genotyping PCR Kit (myPOLS Biotec, Konstanz, Germany) together with an in-house TaqMan primer-probe assay. Validity and specificity of the assay was evaluated using EDTA anti-coagulated whole blood samples and corr...
Background: Adult-type hypolactasia, the physiological decline of lactase some time after weaning, w...
Abstract\ud \ud \ud \ud Background\ud ...
associated with adult-type hypolactasia/lactase persistence among Brazilians of different ethnic gro...
Abstract Primary lactose intolerance has been associated with a single nucleotide polymorphism locat...
Adult-type hypolactasia is a widespread condition throughout the world, causing lactose malabsorptio...
Hos vuxna individer förekommer två fenotyper gällande produktionen av laktas, vilka kallas laktostol...
Adult-type hypolactasia is characterized by the inability to digest lactose during adulthood, due to...
Objectives: To validate C/T-13910 polymorphism associated with primary hypolactasia for clinical pra...
The C/T-13910 mutation is the major factor responsible for the persistence of the lactase-phlorizin ...
Lactose intolerance tends to be one of the most frequent health conditions related to the intake of ...
Lactose intolerance is one of the most common food intolerances. It is caused by decrease of lactase...
PURPOSE: To analyze the usefulness of Quick Lactose Intolerance Test in relation to the genetic test...
CONTEXT: Genotyping of single nucleotide polymorphism (SNP C/T-13910) located upstream of the lactas...
PURPOSE: To analyze the usefulness of Quick Lactose Intolerance Test in relation to the genetic test...
Genetically determined deficiency of the lactase enzyme in adults (primary hypolactasia) is a recess...
Background: Adult-type hypolactasia, the physiological decline of lactase some time after weaning, w...
Abstract\ud \ud \ud \ud Background\ud ...
associated with adult-type hypolactasia/lactase persistence among Brazilians of different ethnic gro...
Abstract Primary lactose intolerance has been associated with a single nucleotide polymorphism locat...
Adult-type hypolactasia is a widespread condition throughout the world, causing lactose malabsorptio...
Hos vuxna individer förekommer två fenotyper gällande produktionen av laktas, vilka kallas laktostol...
Adult-type hypolactasia is characterized by the inability to digest lactose during adulthood, due to...
Objectives: To validate C/T-13910 polymorphism associated with primary hypolactasia for clinical pra...
The C/T-13910 mutation is the major factor responsible for the persistence of the lactase-phlorizin ...
Lactose intolerance tends to be one of the most frequent health conditions related to the intake of ...
Lactose intolerance is one of the most common food intolerances. It is caused by decrease of lactase...
PURPOSE: To analyze the usefulness of Quick Lactose Intolerance Test in relation to the genetic test...
CONTEXT: Genotyping of single nucleotide polymorphism (SNP C/T-13910) located upstream of the lactas...
PURPOSE: To analyze the usefulness of Quick Lactose Intolerance Test in relation to the genetic test...
Genetically determined deficiency of the lactase enzyme in adults (primary hypolactasia) is a recess...
Background: Adult-type hypolactasia, the physiological decline of lactase some time after weaning, w...
Abstract\ud \ud \ud \ud Background\ud ...
associated with adult-type hypolactasia/lactase persistence among Brazilians of different ethnic gro...