The diagnosis of canine distemper is usually based on clinical suspicion of the disease. However, due to the different clinical forms of the disease and other disorders common to clinical signs, the laboratory diagnosis is necessary. The RT-PCR followed by NESTED is currently the method used to diagnosis of CDV (Canine Distemper Virus). The Chapter 2 aimed to standardized the technique of One-Step RT-qPCR-using Syber Green I system. Three commercial kits (Sigma®, Promega® and Qiagen®) were tested and compared. Analytical sensitivity of two detection systems (System A and System B) was determined using a standard curve. The three commercial kits tested detected the virus in 100% of samples from symptomatic dogs and the kit of Qiagen® showed ...
Canine distemper is a highly contagious disease affecting dogs worldwide, often inducing severe neur...
Canine distemper virus is the etiological agent of a severe disease in dogs and many other carnivore...
The purpose of this study was the expression of the conserved region of Canine distemper virus nucle...
ABSTRACT: Three commercial kits of One-Step RT-qPCR were evaluated for the molecular diagnosis of Ca...
O vírus da cinomose canina (CDV) é um patógeno que afeta cães, causando doença grave e que pode leva...
O vírus da cinomose canina (VCC) é um patógeno viral, altamente, contagioso que pode causar doença s...
Empregou-se a técnica de reação em cadeia pela polimerase precedida de transcrição reversa para dete...
The canine distemper virus known as CDV, affects animals carnivorous order and is considered one of ...
A presença do vírus da cinomose canina (CDV) foi avaliada pela reação em cadeia da polimerase, prece...
A presença do vírus da cinomose canina (CDV) foi avaliada pela reação em cadeia da polimerase, prece...
Three commercial kits of One-Step RT-qPCR were evaluated for the molecular diagnosis of Canine Diste...
Diferentes amostras biológicas foram avaliadas (zaragatoa ocular, genital, urina e células mononucle...
Due to changing distemper issues worldwide and to inadequate results of an inter-laboratory study in...
Diferentes amostras biológicas foram avaliadas (zaragatoa ocular, genital, urina e células mononucle...
Urine and leucocytes were comparatively evaluated as clinical samples for ante mortem detection of t...
Canine distemper is a highly contagious disease affecting dogs worldwide, often inducing severe neur...
Canine distemper virus is the etiological agent of a severe disease in dogs and many other carnivore...
The purpose of this study was the expression of the conserved region of Canine distemper virus nucle...
ABSTRACT: Three commercial kits of One-Step RT-qPCR were evaluated for the molecular diagnosis of Ca...
O vírus da cinomose canina (CDV) é um patógeno que afeta cães, causando doença grave e que pode leva...
O vírus da cinomose canina (VCC) é um patógeno viral, altamente, contagioso que pode causar doença s...
Empregou-se a técnica de reação em cadeia pela polimerase precedida de transcrição reversa para dete...
The canine distemper virus known as CDV, affects animals carnivorous order and is considered one of ...
A presença do vírus da cinomose canina (CDV) foi avaliada pela reação em cadeia da polimerase, prece...
A presença do vírus da cinomose canina (CDV) foi avaliada pela reação em cadeia da polimerase, prece...
Three commercial kits of One-Step RT-qPCR were evaluated for the molecular diagnosis of Canine Diste...
Diferentes amostras biológicas foram avaliadas (zaragatoa ocular, genital, urina e células mononucle...
Due to changing distemper issues worldwide and to inadequate results of an inter-laboratory study in...
Diferentes amostras biológicas foram avaliadas (zaragatoa ocular, genital, urina e células mononucle...
Urine and leucocytes were comparatively evaluated as clinical samples for ante mortem detection of t...
Canine distemper is a highly contagious disease affecting dogs worldwide, often inducing severe neur...
Canine distemper virus is the etiological agent of a severe disease in dogs and many other carnivore...
The purpose of this study was the expression of the conserved region of Canine distemper virus nucle...