Apoptosis of Purkinje and Granular Cells of the Cerebellum Following Chronic Ethanol Intake
- Oliveira, Suelen A.
- Chuffa, Luiz Gustavo A.
- Fioruci-Fontanelli, Beatriz Aparecida
- Lizarte Neto, Fermino Sanches
- Novais, Paulo Cezar
- Tirapelli, Luiz Fernando
- Oishi, Jorge Camargo
- Takase, Luiz Fernando
- Stefanini, Maira Aparecida
- Martinez, Marcelo
- Martinez, Francisco Eduardo
DOIAbstract
Ethanol alters motricity, learning, cognition, and cellular metabolism in the cerebellum. We evaluated the effect of ethanol on apoptosis in Golgi, Purkinje, and granule cells of the cerebellum in adult rats. There were two groups of 20 rats: a control group that did not consume ethanol and an experimental group of UChA rats that consumed ethanol at 10 % (<2 g ethanol/kg body weight/day). At 120 days old, rats were anesthetized and decapitated, and their cerebella were collected and fixed. Cerebellar sections were subjected to immunohistochemistry for terminal deoxynucleotide transferase dUTP nick end labeling (TUNEL), caspase-3, X-linked inhibitor of apoptosis protein (XIAP), and insulin-like growth factor 1-receptor (IGF-1R); real-time PC...
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