EFFECT OF CANARIUMODONTOPHYLLUM STEM BARK EXTRACT AGAINST HUMAN COLORECTAL CANCER CELL LINE HCT 116

Canarium odontophyllumstem bark was proven to cause cytotoxicity on colorectal cancer cell line HCT116 by inducing cell death via apoptosis. We aimed to assess cytotoxic mechanism of the acetone extract from stem bark of C. odontophyllum against HCT 116. The IC50 value of extract against HCT 116 was determined using MTT assay for 48 hours of treatment. ROS assay with dihydroethidium staining was conducted for 30 minutes, 1 hour and 2 hours. The DNA integrity of HCT 116 was evaluated using alkaline comet assay. The extract showed cytotoxic effect on HCT 116 after 48 hours of treatment with IC50 value of 82.0 ± 9.3 µg/mL. At 2 hours of post-treatment, the extract induced elevation of ROS level in the cell lines compared to control at 16.0 ± 7.2 % and 10.0 ± 0.9 %, respectively. Elevated tail moment and percentage of DNA in tail revealed DNA damage when the cells were incubated to low doses of extract at IC10 and IC25 values. Acetone extract from C.odontophyllumstem bark has exerted cytotoxic activity against HCT 116 by increasing ROS level and interfering DNA integrity of the cell line therefore, has the potential to be developed as anticancer agent