Entrapment and growth of <i>Chlamydomonas reinhardtii</i> in biocompatible silica hydrogels

Homburg SV, Venkanna D, Kraushaar K, Kruse O, Kroke E, Patel A. Entrapment and growth of &lt;i&gt;Chlamydomonas reinhardtii&lt;/i&gt; in biocompatible silica hydrogels. Colloids and Surfaces B: Biointerfaces. 2018;173:233-241.In this work, we aimed at improved viability and growth of the microalga *Chlamydomonas reinhardtii* in transparent silica hydrogels based on low-ethanol, low-sodium and low-propylamine synthesis. Investigation into replacement of conventional base KOH by buffers dipotassium phosphate and tris(hydroxymethyl)aminomethane along with increased precursor concentrations yielded an aqueous synthesis route which provided a gelation within 10 min, absorptions below 0.1 and elastic moduli of 0.04-4.23 kPa. The abrasion resistance enhanced by 41 % compared to calcium alginate hydrogels and increased to 70-85 % residual material on addition of chitosan. Entrapment of microalgae in low-sodium and low-propylamine silica hydrogels maintained the PSII quantum yield above 0.3 and growth rates of 0.23 ± 0.01 d-1, similarly to cells entrapped in calcium alginate. These promising results pave the way for the entrapment of sensitive, photosynthetically active and growing cells for in robust biotechnological applications