SINTESIS SENYAWA 1-(3-ASETIL-2,4,6-TRIHIDROKSIFENIL) ETANON (DAPG) DAN UJI SITOTOKSISITAS TERHADAP SEL KANKER SERVIKS, KANKER KOLON DAN KANKER PAYUDARA

SINTESIS SENYAWA 1-(3-ASETIL-2,4,6-TRIHIDROKSIFENIL) ETANON (DAPG) DAN UJI SITOTOKSISITAS TERHADAP SEL KANER SERVIKS, KANKER KOLON DAN KANKER PAYUDARA TATA LUGAS NASTITI Program Studi Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sebelas Maret ABSTRAK Senyawa 1-(3-asetil-2,4,6-trihidroksifenil) etanon (DAPG) merupakan senyawa turunan ploroglusinol telah berhasil disintesis dengan mereaksikan ploroglusinol dengan asam asetat anhidrid menggunakan katalis asam metasulfonat melalui reaksi esterifikasi dan penataan ulang Fries. Identifikasi senyawa tersebut dikarakterisasi dengan uji titik leleh, spektroskopi FT-IR, dan 1D NMR dan diperoleh rendemen sebesar 34%. Uji sitotoksisitas senyawa DAPG dilakukan pada seri konsentrasi 10; 15; 20; 25; 50; 75; 90; dan 100 µg/mL terhadap sel kanker HeLa, WiDr dan MCF7. Hasil uji sitotoksisitas menunjukkan DAPG hanya aktif terhadap sel HeLa dengan nilai IC50 sebesar 50,1 µg/mL dan viabilitas sel sebesar 58,73%. Kata kunci: 1-(3-asetil-2,4,6-trihidroksifenil)etanon (DAPG), IC50, sitotoksisitas, sel HeLa, sel WiDr dan sel MCF7 SYNTHESIS OF 1-(3-ACETYL-2,4,6-TRIHYDROXYPHENYL) ETHANONE (DAPG) COMPOUND AND ITS CYTOTOXICITY OF CERVIX CANCER, COLON CANCER AND BREAST CANCER CELLS TATA LUGAS NASTITI Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Sebelas Maret ABSTRACT 1-(3-acetyl-2,4,6-trihydroxyphenyl) ethanone (DAPG) compounds had been successfully synthesized using commercially available phloroglucinol, acetate anhydride and methanesulfonic acid as a catalyst through esterification reaction and Fries rearrangement. The synthesized product, DAPG compound were characterized by FT-IR, NMR spectroscopy which were gave 34% yield. Cytotoxicity assay of DAPG compound had been measured in 10; 15; 20; 25; 50; 75; 90; dan 100 µg/mL of concentration against HeLa, WiDr and MCF7 cancer cells. DAPG compound had showed better bioactivity only on HeLa cancer cells with respectively gave 50,1 µg/mL of IC50 values and 58,73% of its cell viabillity. Keywords: 1-(3-acetyl-2,4,6-trihydroxyphenyl) ethanone (DAPG), cytotoxicity, IC50, HeLa, MCF7, and WiDr cancer cells