Study of autologour transplantation of adipore devived stem cell in sein graft survival in non diabetic and diabetic rats and correlation with VEGF and TGFb3

Background: Skin grafts are frequently used for a variety of indications in plastic andreconstructive surgery. heir necrosis is a common complication while differenttherapies have been proposed. Currently, adipose-derived stem cells (ASCs) holdgreat promise for their angiogenic potential and role during tissue repair. In this study,autologous transplantation of ASCs was usedin skin grafts in diabetic and nondiabeticrats to determine if it increases angiogenesis, skin graft survival and woundhealing.Methods: ASCs were isolated, cultured, labeled with fluorescent dye and injectedunder full thickness skin grafts in twenty rats (group 1 and group 3, non-diabetic anddiabetic respectively, ten rats in each group), while twenty others served as controls(group 2 and group 4, non-diabetic and diabetic respectively, ten rats in each group).immunohistochemistry. Additionally, immunohistochemical staining intensity ofvascular endothelial growth factor (VEGF) and transforming growth factor b3(TGFb3) was assessed in all grafts.Results: Mean area of graft necrosis was significantly less in group 1 than group 2(6.12% vs. 32.62%, p<0.01). Statistically significant increase of microvessel density,collagen density, VEGF and TGFb3 expression was noted in group 1 compared withgroup 2 (all: p<0.01). Additionally, mean area of graft necrosis was significantly lessin group 3 than group 4 (7.49% vs. 39.67%, p