PURIFICATION AND STUDY OF THE PROPERTIES OF A B-GLUCOSIDASE AND A B-GALACTOSIDASE FROM BARLEY SEEDS. APPLICATION OF THE ENZYMES ON THE HYDROLYSIS OF LACTOSE IN MILK AND ITS PRODUCTS

TWO ENZYMES A B-GLUCOSIDASE AND A B-GALACTOSIDASE WERE ISOLATED AND CHARACTERIZED FROM BARLEY SEEDS. THE B-GLUCOSIDASE IS A SINGLE BASIC POLYPEPTIDE (PI>8,5) WITH A MR OF 53.000 ACTING OPTIMALLY AT PH 4.5-5.0. THE B-GALACTOSIDASE IS COMPOSED OF TWO SUBUNITS WITH A MR OF 42.000 AND 33.000 RESPECTIVELY, IS ACIDIC IN NATURE (PI8,5), ΜΕ Μ.Β. 53.000 ΚΑΙ ΔΡΑ ΒΕΛΤΙΣΤΑ ΣΕ PH 4,5-5,0. Η Β-ΓΑΛΑΚΤΟΣΙΔΑΣΗ ΑΠΟΤΕΛΕΙΤΑΙ ΑΠΟ ΔΥΟ ΥΠΟΜΟΝΑΔΕΣ ΜΕ Μ.Β. 42.000 ΚΑΙ 33.000 ΑΝΤΙΣΤΟΙΧΑ, ΕΙΝΑΙ ΟΞΙΝΗ (ΡΙ