Add to ORKGEnzymes can be tailored for optimal performance in industrial applications by directing their evolution in vitro. This approach is particularly attractive for engineering industrial enzymes. We have created an efficient para-nitrobenzyl esterase over six generations of random point mutagenesis and recombination couled with screening for improved variants. The best clones identified after four generations of sequential random mutagenesis and two generations of random recombination display more than 150 times the p-nitrobenzyl esterase activity of wild type towards loracarbef-p-nitrobenzyl ester in 15% dimethylformamide. Although the contributions of individual effective amino acid substitutions to enhanced activity are small (<2-fold increas...
Abstract: Provided that the industrial constraints have been properly defined, the directed evolutio...
Since the fIrst site directed mutagenesis in the mid 1980s, protein engineering has become a very po...
Occasionally, researchers need to modify enzymes through amino acid substitutions to make them more ...
Through sequential generations of random mutagenesis and screening, we have directed the evolution o...
Sets of genes improved by directed evolution can be recombined in vitro to produce further improveme...
The goal of this research is to engineer enzymes to be efficient and economically attractive catalys...
Although enzymes are the product of millions of years of natural evolution, their properties are fre...
The ease with which enzymes can be adapted from their native roles and engineered to function specif...
Abstract The engineering of enzymes with altered activity, specificity and stability, using directed...
Directed evolution has emerged in just a few years as one of the most effective approaches to adapti...
The ease with which enzymes can be adapted from their native roles and engineered to function specif...
ABSTRACT: The ease with which enzymes can be adapted from their native roles and engineered to funct...
Directed evolution has become a full-grown tool in molecular biology nowadays. The methods that are ...
Enzymes have been long used in man-made biochemical processes, from brewing and fermentation to curr...
Directed evolution has become a full-grown tool in molecular biology nowadays. The methods that are ...
Abstract: Provided that the industrial constraints have been properly defined, the directed evolutio...
Since the fIrst site directed mutagenesis in the mid 1980s, protein engineering has become a very po...
Occasionally, researchers need to modify enzymes through amino acid substitutions to make them more ...
Through sequential generations of random mutagenesis and screening, we have directed the evolution o...
Sets of genes improved by directed evolution can be recombined in vitro to produce further improveme...
The goal of this research is to engineer enzymes to be efficient and economically attractive catalys...
Although enzymes are the product of millions of years of natural evolution, their properties are fre...
The ease with which enzymes can be adapted from their native roles and engineered to function specif...
Abstract The engineering of enzymes with altered activity, specificity and stability, using directed...
Directed evolution has emerged in just a few years as one of the most effective approaches to adapti...
The ease with which enzymes can be adapted from their native roles and engineered to function specif...
ABSTRACT: The ease with which enzymes can be adapted from their native roles and engineered to funct...
Directed evolution has become a full-grown tool in molecular biology nowadays. The methods that are ...
Enzymes have been long used in man-made biochemical processes, from brewing and fermentation to curr...
Directed evolution has become a full-grown tool in molecular biology nowadays. The methods that are ...
Abstract: Provided that the industrial constraints have been properly defined, the directed evolutio...
Since the fIrst site directed mutagenesis in the mid 1980s, protein engineering has become a very po...
Occasionally, researchers need to modify enzymes through amino acid substitutions to make them more ...