Nonlinear microscopy methods for imaging and particle tracking in thick biological specimen
- Perillo, Evan Paul
DOIAbstract
Optical microscopy techniques such as single-particle tracking and high-resolution (<500 nm) imaging are critical tools for the advancement of biological research. However most high-resolution optical techniques utilize a camera-based or confocal-based detection scheme, which limits the working distance into samples to approximately 10 μm due to light scattering. Nonlinear excitation methods, such as two- and three-photon microscopy, have enabled imaging in thick and scattering samples due to their longer excitation wavelengths and absence of spatial filtering. However, nonlinear excitation is rarely utilized for single-particle tracking as it traditionally offers slightly worse resolution than the aforementioned methods. This dissertation ...
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