In many protein kinases, the phosphorylation of one or more residues in the activation segment is critical for driving the conformational change, which allows the activation of the kinase. The yeast checkpoint kinase Rad53 presents two phosphorylatable Threonine residues (T354 and T358) in the activation segment. In this thesis I have generated rad53 mutants with affected activation segment functions resulting from Alanine or Aspartate substitution of single or both the T354 and T358. These results pinpointed functional regulatory roles of the phosphorylations of both Threonine residues in the catalytic activation of Rad53. Interestingly, the rad53-T354D-T358D mutation caused the accumulation of the S129-phosphorylated isoform of the histon...
The complex molecular biology involved in gastrointestinal stromal tumors (GIST) progressive disease...
Transposable Elements (TEs) are a class of repetitive DNA sequences able to mobilize and change loca...
Using photoemission spectroscopy (PES) the interface between the amino acid L-cysteine and a Au subs...
G-protein-coupled receptors (GPCRs) represent, nowadays, one of the most productive source of drug t...
The urokinase-type plasminogen activator receptor (uPAR), is a multifunctional protein with an impre...
TP53 is an essential tumor suppressor gene which is inactivated in every second tumor. Most frequent...
The mitotic spindle assembly checkpoint (SCP) is a signal transduction pathway that ensures proper c...
Aldose Reductase (AR) is the first enzyme of the 'sorbitol pathway'. It is an NADPH dependent enzyme...
In liver disease, irregular proliferation of hepatocytes is an important factor favoring hepatocarci...
This project addressed a variety of aspects of the bacterial transposon Tn7. Firstly, the known sequ...
Apple Proliferation (AP) is a phytoplasma-related associated with 'Candidatus Phytoplasma mali' ('Ca...
Panulirus argus Virus 1 (PaV1) is an emerging disease in Caribbean spiny lobster Panulirus argus. It...
Glucocorticoids are hormones with a vital role in regulation of metabolic and defence responses. The...
Neurotransmitter and hormon release is based on Ca2+ dependent exocytosis. The sequential...
Phenylketonuria (PKU) is an inborn error of metabolism involving the enzyme phenylalanine (L-Phe) hy...
The complex molecular biology involved in gastrointestinal stromal tumors (GIST) progressive disease...
Transposable Elements (TEs) are a class of repetitive DNA sequences able to mobilize and change loca...
Using photoemission spectroscopy (PES) the interface between the amino acid L-cysteine and a Au subs...
G-protein-coupled receptors (GPCRs) represent, nowadays, one of the most productive source of drug t...
The urokinase-type plasminogen activator receptor (uPAR), is a multifunctional protein with an impre...
TP53 is an essential tumor suppressor gene which is inactivated in every second tumor. Most frequent...
The mitotic spindle assembly checkpoint (SCP) is a signal transduction pathway that ensures proper c...
Aldose Reductase (AR) is the first enzyme of the 'sorbitol pathway'. It is an NADPH dependent enzyme...
In liver disease, irregular proliferation of hepatocytes is an important factor favoring hepatocarci...
This project addressed a variety of aspects of the bacterial transposon Tn7. Firstly, the known sequ...
Apple Proliferation (AP) is a phytoplasma-related associated with 'Candidatus Phytoplasma mali' ('Ca...
Panulirus argus Virus 1 (PaV1) is an emerging disease in Caribbean spiny lobster Panulirus argus. It...
Glucocorticoids are hormones with a vital role in regulation of metabolic and defence responses. The...
Neurotransmitter and hormon release is based on Ca2+ dependent exocytosis. The sequential...
Phenylketonuria (PKU) is an inborn error of metabolism involving the enzyme phenylalanine (L-Phe) hy...
The complex molecular biology involved in gastrointestinal stromal tumors (GIST) progressive disease...
Transposable Elements (TEs) are a class of repetitive DNA sequences able to mobilize and change loca...
Using photoemission spectroscopy (PES) the interface between the amino acid L-cysteine and a Au subs...