An Efficient Benchtop System for Multigram-Scale Kinetic Resolutions Using Aldolase Antibodies

The preparative scale kinetic resolution of racemic aldols 1‐4 using aldolase antibodies 38C2 (Aldrich no. 47 995–0) and 84G3 (Aldrich no. 52 785–8) is described. These reactions use a biphasic aqueous/organic solvent system that allows the catalyst to be reused. Reaction scales range from miligrams to grams, with 0.0086 to 0.12 mol % of antibody binding sites. Because antibodies 38C2 and 84G3 have opposite enantioselectivities, both aldol product enantiomers are accessible by kinetic resolution