Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-Å resolution
- Armache, J.
- Jarasch, A.
- Anger, A.
- Villa, E.
- Becker, T.
- Bhushan, S.
- Jossinet, F.
- Habeck, M.
- Dindar, G.
- Franckenberg , S.
- Marguez, V.
- Mielke, T.
- Thomm, M.
- Berninghausen, O.
- Beatrix, B.
- Söding, J.
- Westhof, E.
- Wilson, D.
- Beckmann, R.
DOIAbstract
Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum aestivum) 80S ribosome at 5.5-Å resolution. This map, together with a 6.1-Å map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model ∼98 of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES–ES and r-protein–ES interactions, providing insight into the structure and evolution of ...
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