BACKGROUND: The sequencing of immunoglobulin (Ig) transcripts from single B cells yields essential information about Ig heavy:light chain pairing, which is lost in conventional bulk sequencing experiments. The previously limited throughput of single-cell approaches has recently been overcome by the introduction of multiple next-generation sequencing (NGS)-based platforms. Furthermore, single-cell techniques allow the assignment of additional data types (e.g. cell surface marker expression), which are crucial for biological interpretation. However, the currently available computational tools are not designed to handle single-cell data and do not provide integral solutions for linking of sequence data to other biological data. RESULTS: Here w...
High-throughput sequencing of immunoglobulin (Ig) repertoires (Ig-seq) is a powerful method for quan...
Future successful exploitation of antibodies as diagnostic and therapeutic agents will greatly benef...
In human immune system, V(D)J recombination produces an enormously large repertoire of immunoglobuli...
BACKGROUND: The sequencing of immunoglobulin (Ig) transcripts from single B cells yields essential i...
Single-cell PCR and sequencing of full-length Ig heavy (Igh) and Igk and Igl light chain genes is a ...
Over the last few years, there has been a rapid expansion in the application of information technolo...
The diversity of the immunological repertoire has long been a subject of research focus, providing i...
T-cell specificity is determined by the T-cell receptor, a heterodimeric protein coded for by an ext...
Adaptive immune repertoires are composed by the ensemble of B and T cell receptors (BCR, TCR) within...
Next-generation (high throughput) DNA sequencing of immunoglobulin variable region and T-cell recept...
In recent years there has been a rapid increase in the use of single-cell sequencing (scRNA-seq) app...
Somatic assembly of T cell receptor and B cell receptor (BCR) genes produces a vast diversity of lym...
Each B-cell receptor consists of a pair of heavy and light chains. High-throughput sequencing can id...
Single-cell sequencing is an emerging technology in the field of immunology and oncology that allows...
Background Deep-sequencing methods are rapidly developing in the field of B-cell receptor (BCR) and ...
High-throughput sequencing of immunoglobulin (Ig) repertoires (Ig-seq) is a powerful method for quan...
Future successful exploitation of antibodies as diagnostic and therapeutic agents will greatly benef...
In human immune system, V(D)J recombination produces an enormously large repertoire of immunoglobuli...
BACKGROUND: The sequencing of immunoglobulin (Ig) transcripts from single B cells yields essential i...
Single-cell PCR and sequencing of full-length Ig heavy (Igh) and Igk and Igl light chain genes is a ...
Over the last few years, there has been a rapid expansion in the application of information technolo...
The diversity of the immunological repertoire has long been a subject of research focus, providing i...
T-cell specificity is determined by the T-cell receptor, a heterodimeric protein coded for by an ext...
Adaptive immune repertoires are composed by the ensemble of B and T cell receptors (BCR, TCR) within...
Next-generation (high throughput) DNA sequencing of immunoglobulin variable region and T-cell recept...
In recent years there has been a rapid increase in the use of single-cell sequencing (scRNA-seq) app...
Somatic assembly of T cell receptor and B cell receptor (BCR) genes produces a vast diversity of lym...
Each B-cell receptor consists of a pair of heavy and light chains. High-throughput sequencing can id...
Single-cell sequencing is an emerging technology in the field of immunology and oncology that allows...
Background Deep-sequencing methods are rapidly developing in the field of B-cell receptor (BCR) and ...
High-throughput sequencing of immunoglobulin (Ig) repertoires (Ig-seq) is a powerful method for quan...
Future successful exploitation of antibodies as diagnostic and therapeutic agents will greatly benef...
In human immune system, V(D)J recombination produces an enormously large repertoire of immunoglobuli...