Styryl (FM) dyes have been used for more than two decades to investigate exo- and endocytosis in conventional synapses. However, they are difficult to use in the inner hair cells of the auditory pathway (IHCs), as FM dyes appear to penetrate through mechanotransducer channels into the cytosol of IHCs, masking endocytotic uptake. To solve this problem we applied to IHCs the FM dye photo-oxidation technique, which renders the dyes into electron microscopy markers. Photo-oxidation allowed the unambiguous identification of labeled organelles, despite the presence of FM dye in the cytosol. This enabled us to describe the morphologies of several organelles that take up membrane in IHCs, both at rest and during stimulation. At rest, endosome-like ...
Hair cells in mouse cochlear cultures are selectively labeled by brief exposure to FM1-43, a styryl ...
In small presynaptic boutons in brain, synaptic vesicles are thought not to merge with the plasma me...
AbstractThe fluorescent probe FM1-43 has been used extensively for imaging vesicle recycling; howeve...
Styryl (FM) dyes have been used for more than two decades to investigate exo- and endocytosis in con...
Styryl (FM) dyes have been used for more than two decades to investigate exo- and endocytosis in con...
The molecular composition of the organelles involved in membrane recycling is difficult to establish...
The ways in which cell architecture is modelled to meet cell function is a poorly understood facet o...
Hair cells in mouse cochlear cultures are selectively labeled by brief exposure to FM1-43, a styryl ...
AbstractStyryl dyes (also referred to as FM dyes) become highly fluorescent upon binding to membrane...
Previous work has shown that the fluorescent styryl dye FM1-43 stains nerve terminals in an activity...
AbstractApical membrane recycling has been proposed to be important for normal hair cell function. T...
Synaptic vesicle recycling sustains high rates of neurotransmission at the ribbon-type active zones ...
To maintain transmitter release during intense stimulation, neurons need to efficiently recycle vesi...
<p>(<b>A</b>) Experimental outline: IHCs incubated with FM 1-43 take up the dye (red symbols) in end...
AbstractPerhaps synaptic vesicles can recycle so rapidly because they avoid complete exocytosis, and...
Hair cells in mouse cochlear cultures are selectively labeled by brief exposure to FM1-43, a styryl ...
In small presynaptic boutons in brain, synaptic vesicles are thought not to merge with the plasma me...
AbstractThe fluorescent probe FM1-43 has been used extensively for imaging vesicle recycling; howeve...
Styryl (FM) dyes have been used for more than two decades to investigate exo- and endocytosis in con...
Styryl (FM) dyes have been used for more than two decades to investigate exo- and endocytosis in con...
The molecular composition of the organelles involved in membrane recycling is difficult to establish...
The ways in which cell architecture is modelled to meet cell function is a poorly understood facet o...
Hair cells in mouse cochlear cultures are selectively labeled by brief exposure to FM1-43, a styryl ...
AbstractStyryl dyes (also referred to as FM dyes) become highly fluorescent upon binding to membrane...
Previous work has shown that the fluorescent styryl dye FM1-43 stains nerve terminals in an activity...
AbstractApical membrane recycling has been proposed to be important for normal hair cell function. T...
Synaptic vesicle recycling sustains high rates of neurotransmission at the ribbon-type active zones ...
To maintain transmitter release during intense stimulation, neurons need to efficiently recycle vesi...
<p>(<b>A</b>) Experimental outline: IHCs incubated with FM 1-43 take up the dye (red symbols) in end...
AbstractPerhaps synaptic vesicles can recycle so rapidly because they avoid complete exocytosis, and...
Hair cells in mouse cochlear cultures are selectively labeled by brief exposure to FM1-43, a styryl ...
In small presynaptic boutons in brain, synaptic vesicles are thought not to merge with the plasma me...
AbstractThe fluorescent probe FM1-43 has been used extensively for imaging vesicle recycling; howeve...