Cloning, pharmacological characteristics and expression pattern of the rat GABAA receptor α4 subunit
- Wisden, W.
- Herb, A.
- Wieland, H.
- Keinänen, K.
- Lüddens, H.
- Seeburg, P.
DOIAbstract
A cDNA of rat brain encoding the GABAA receptor α4 subunit has been cloned. Recombinant receptors composed of α4, β2 and γ2 subunits bind with high affinity the GABA agonist [3H]muscimol and the benzodiazepine ‘alcohol antagonist’ [3H]Ro 15-4513, but fail to bind benzodiazepine agonists. The α4 subunit is expressed mainly in the thalamus, as assessed by in situ hybridization histochemistry, and may participate in a major population of thalamic GABAA receptors. The α4 mRNA is found at lower levels in cortex and caudate putamen, and is rare in cerebellum
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