Add to ORKGTandem affinity purification (TAP) is a generic two-step affinity purification protocol for isolation of TAP-tagged proteins together with associated proteins. We used bacterial artificial chromosome to heterologously express TAP-tagged murine Sgo1 protein in human HeLa cells. This allowed us to test the functionality of the Sgo1-TAP protein by RNA interference-mediated depletion of the endogenous human Sgo1. Here, we present an optimized protocol for purification of TAP-tagged Sgo1 protein as well as KIAA1387 from HeLa cells with detailed instructions. The purification protocol can be completed in 1 day and it should be applicable to other proteins
Identification of protein interactors is fundamental to understanding their functions. Here, we desc...
Multi-protein complexes, rather than single proteins acting in isolation, often govern molecular pat...
Multi-protein complexes, rather than single proteins acting in isolation, often govern molecular pat...
Tandem affinity purification (TAP) is a generic two-step affinity purification protocol for isolatio...
Tandem affinity purification (TAP) is a generic two-step affinity purification protocol for isolatio...
Isolation and dissection of native multiprotein complexes is a central theme in functional genomics....
Isolation and dissection of native multiprotein complexes is a central theme in functional genomics....
In recent years, several methods have been developed to analyze protein-protein interactions under n...
Tandem affinity purification (TAP) is a generic two-step affinity purification protocol that enables...
Contains fulltext : 51868.pdf (publisher's version ) (Closed access)Isolation and ...
Identification of protein-protein interactions is essential for elucidating biochemical mechanism of...
Tandem affinity purification (TAP) has been widely used for the analysis of protein complexes. We in...
Tandem affinity purification (TAP) has been widely used for the analysis of protein complexes. We in...
Tandem Affinity Purification (TAP) is a technique based on attaching a tag to the N- or C- terminus ...
It has become clear in recent years that the G beta gamma subunits of heterotrimeric proteins serve ...
Identification of protein interactors is fundamental to understanding their functions. Here, we desc...
Multi-protein complexes, rather than single proteins acting in isolation, often govern molecular pat...
Multi-protein complexes, rather than single proteins acting in isolation, often govern molecular pat...
Tandem affinity purification (TAP) is a generic two-step affinity purification protocol for isolatio...
Tandem affinity purification (TAP) is a generic two-step affinity purification protocol for isolatio...
Isolation and dissection of native multiprotein complexes is a central theme in functional genomics....
Isolation and dissection of native multiprotein complexes is a central theme in functional genomics....
In recent years, several methods have been developed to analyze protein-protein interactions under n...
Tandem affinity purification (TAP) is a generic two-step affinity purification protocol that enables...
Contains fulltext : 51868.pdf (publisher's version ) (Closed access)Isolation and ...
Identification of protein-protein interactions is essential for elucidating biochemical mechanism of...
Tandem affinity purification (TAP) has been widely used for the analysis of protein complexes. We in...
Tandem affinity purification (TAP) has been widely used for the analysis of protein complexes. We in...
Tandem Affinity Purification (TAP) is a technique based on attaching a tag to the N- or C- terminus ...
It has become clear in recent years that the G beta gamma subunits of heterotrimeric proteins serve ...
Identification of protein interactors is fundamental to understanding their functions. Here, we desc...
Multi-protein complexes, rather than single proteins acting in isolation, often govern molecular pat...
Multi-protein complexes, rather than single proteins acting in isolation, often govern molecular pat...