Directed mutagenesis alters the stereochemistry of catalysis by isolated ketoreductase domains from the erythromycin polyketide synthase
- Baerga-Ortiz, A.
- Popovic, B.
- Siskos, A.
- O'hare, H.
- Spiteller, D.
- Williams, M.
- Campillo, N.
- Spencer, J.
- Leadlay, P.
DOIAbstract
The ketoreductase (KR) domains eryKR(1) and eryKR(2) from the erythromycin-producing polyketide synthase (PKS) reduce 3-ketoacyl-thioester intermediates with opposite stereospecificity. Modeling of eryKR(1) and eryKR(2) showed that conserved amino acid
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